The 224th was a division of clone troopers serving within the Grand Army of the Republic as the Clone Wars began. Within this unit were troopers who were famously known as the Mud Jumpers. The 224th participated in a battle that took place on the planet of Mimban in 22 BBY.
During this battle, the MudJumpers of the 224th were recognized by the Bettie-Bot host of a musical show on the HoloNet's Grand Army of the Republic broadcast channel. This shout-out, which included dedicating a song, was overheard by other clone troopers named Echo, Hevy, Fives, Nub, Droidbait, and Cutup, who were stationed at a Republic listening post located on the moon of the planet Rishi.
The first mention of the 224th occurred in "Rookies," the fifth episode of Star Wars: The Clone Wars animated television series' Season One, which debuted on October 24, 2008.