The conflict known as the Battle of Mos Espa, alternatively called the Battle for Mos Espa, the Tatooine Showdown, or the Mos Espa Territory Dispute, transpired between the Fett gotra and the Pyke Syndicate during the war against the Pykes.
Following the Pyke Syndicate's bombing of Garsa Fwip's Sanctuary, Boba Fett, along with his enforcers Fennec Shand, Din Djarin, Krrsantan, his pair of Gamorrean bodyguards, and the Mods, strategically positioned themselves around the city of Mos Espa to brace for the impending combat. Fett, Shand, and Djarin established a defensive line amidst the Sanctuary's ruins, anticipating the imminent arrival of reinforcements from the citizens of Freetown, as Cobb Vanth stood against the spice trade.
Fett was soon met by his former mentor Cad Bane, who was acting on behalf of the Pykes to convince Fett to surrender and allow the Pykes to continue the spice trade on Tatooine. He informed Fett that he had dealt with the people of Freetown and their sheriff, telling him that there was no one coming to help him. He also revealed that the Pykes were responsible for the massacre of the Tusken Raider tribe that had taken Fett in after his escape for the sarlacc pit. Fett maintained his composure and asserted to Bane that the Pyke presence would not be tolerated. Bane departed, warning that refusal to surrender would result in the annihilation of Fett and his forces.
Fett contemplated his options with Shand and Djarin, but they promptly received multiple urgent transmissions from their other gotra forces, revealing that the three crime families, with whom Fett had brokered a truce, had betrayed them. These families secretly opposed Fett's rule due to his restrictions on their revenue streams and their desire to exploit the power vacuum that would arise from his elimination.
The Aqualish family engaged the Mods in the alleyways of Mos Espa, the Trandoshan family battled Krrsantan in the streets, while the Klatooinians assassinated Boba's Gamorrean guards in the upper sprawl. Upon learning of the situation, Fett inquired with Shaiz's majordomo about the syndicate's operational base. The majordomo attempted to evade the question, prompting Shand to threaten him with a blaster shot, compelling him to reveal that they were operating out of the Desert Survey Office in Mos Eisley. Fett instructed Shand to proceed there to assassinate them, while he and the others would defend against the syndicate's assault.
Krrsantan initially faced overwhelming numbers of Trandoshans, and the Mods were nearly overrun by the Aqualish, but Shand responded to their distress call and eliminated most of the Aqualish attackers, forcing them to retreat. Shand then directed the surviving Mods to head to Sanctuary before leaving the town. Similarly, Krrsantan triumphed over the Trandoshans and proceeded to Sanctuary.
Back at Sanctuary, the Pykes emerged, with an Pyke capo demanding Fett's surrender. The majordomo offered to surrender in Fett's place, and he agreed, writing what he thought was surrender terms. When he came out to meet the capo, he only then discovered that Fett did not make any concessions and tried to change the subject to no avail. Fett and Djarin then appeared, having used the majordomo as a distraction. They slew many Pykes, and while they managed to get a several shots on them, their beskar armor protected them from serious injuries, though they were nearly overwhelmed from the sheer amount of enemies. However, the Pykes were forced to retreat when the people of Freetown came to help them along with Fett's remaining forces; the people of Freetown had been angered at how Vanth was shot in cold blood and knew that the spice trade would harm the planet.
The Pykes then deployed two Scorpenek annihilator droids to resume their offensive. Due to the droids' shielding and wanting to avoid collateral damage to the town, Fett left for his palace to get his rancor while tasking Djarin with protecting their forces. Djarin managed to distract one droid while another went for the remaining forces. Fett returned with his rancor and with the help of Djarin and Grogu, managed to destroy the droid chasing them. Meanwhile, the Mods and Freetown forces managed to inflict sizeable casualties onto the Pykes before Fett arrived and destroyed the other droid.
Fett and the rancor proceeded to kill a few more Pykes before Bane appeared and scared the rancor off with his flamethrowers. Bane initially had the upper hand on Fett, but Fett was able to recover and slew him with his Gaderffii stick. Djarin tried to calm Fett's rancor down, who had gone on a rampage and was further exacerbated by the Mods and Freetown forces firing on it. He was unsuccessful in doing so, but Grogu managed to use The Force to lull the rancor into sleep, concluding the battle.